3D Analysis of the Myocardial Microstructure: Determination by Johanna Maria Ticar

By Johanna Maria Ticar

The grasp thesis of Johanna Maria Ticar unearths high-resolution insights into the myocardial microstructure and illustrates that cardiac muscle fibers are directly, operating in parallel with one most well liked fiber course, even though, deposits reminiscent of fats appear to compromise the commonplace and compact constitution. moment harmonic iteration imaging mixed with optical tissue clearing is a correct strategy for deciding on the three-d muscle fiber and sheet orientations and consequently, permits the calculation of fiber rotation in the course of the ventricle wall.

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Extra resources for 3D Analysis of the Myocardial Microstructure: Determination of Fiber and Sheet Orientations

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Hence, 2000 images were used in order to volume-render a sample of a depth of 800 μm. The cardiac muscle fibers are parallel and densely packed. Cleavage planes that separate individual sheets are visible and indicated by the arrows. g. (Rohmer et al. , 2006)), sheets are 3-4 cells wide, while the sheets observable in this 3-D reconstruction seem to exceed this width. 5: Depiction of volume-rendered optical sections aquired by SHG imaging. 4 μm leading to a total depth of 800 μm. Due to the computational burden, the image stack was cropped in x- and y-direction to 330 and 290 μm, respectively.

However, the quantity of samples from the right ventricle tested was minimum, which did not allow for any conclusions to be drawn. 2: Data from two heart samples from the right ventricle under specification of the original position (RV = right ventricle, end = endocardial, med = medial, epi = epicardial) of the sample, the respective depth, the rotation per depth and mm depth, the concentration parameter b, the coefficient of determination R2 , and the dispersion parameter κ. ). Sample no. XXI XXI Stack a b Heart no.

2006). 1 shows the results from the heart samples of the left ventricle that were either biaxially or triaxially tested before observing the microstructure. The table includes the original position of the samples in the heart (specified by left ventricle (LV), endocardial (end), medial (med) and epicardial (epi)), the depth of the respective heart samples, as well as the computed rotation Φ per depth. The concentration parameter b, the coefficient of determination R2 and the dispersion parameter κ were calculated for each image in the z-stack, the mean values of which are listed in the table for each of the 29 specimen.

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