Advanced Fluorescence Microscopy: Methods and Protocols by Peter J. Verveer

By Peter J. Verveer

This quantity offers an outline of complicated fluorescence microscopy, overlaying a wide variety of equipment. every one bankruptcy specializes in a unique procedure and offers a realistic consultant for program in organic platforms. Written within the hugely winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and pointers on troubleshooting and fending off recognized pitfalls.

Authoritative and state-of-the-art, Advanced Fluorescence Microscopy: tools and Protocols seeks to supply scientists with tools for organic platforms which are of curiosity.

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It helps to have the temperature probe immersed in water in the incubator as this gives a better indicator of the temperature in the dish being imaged and is also a more constant temperature so the heater will not be switching on and blowing on your samples every time there is a slight dip in temperature. Any dirt or liquid on the coverslip area of the dish can hinder good TIRF imaging. Clean the coverslip with ethanol and ensure it is dry before imaging. Also make sure the imaging dish is flat and there is enough oil.

Schmoranzer J, Goulian M, Axelrod D et al (2000) Imaging constitutive exocytosis with total internal reflection fluorescence microscopy. Burridge K, Fath K, Kelly T et al (1988) Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol 4:487–525 52. Burridge K, Chrzanowska-Wodnicka M (1996) Focal adhesions, contractility, and signaling. Webb DJ, Parsons JT, Horwitz AF (2002) Adhesion assembly, disassembly and turnover in migrating cells-over and over and over again.

Poulter et al. Grigoriev I, Splinter D, Keijzer N et al (2007) Rab6 regulates transport and targeting of exocytotic carriers. Berginski ME, Vitriol EA, Hahn KM et al (2011) High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells. PLoS One 6:e22025 12. Partridge MA, Marcantonio EE (2006) Initiation of attachment and generation of mature focal adhesions by integrin-containing filopodia in cell spreading. Fletcher SJ, Poulter NS, Haining EJ et al (2011) Clathrin‐mediated endocytosis regulates occludin, and not focal adhesion, distribution during epithelial wound healing.

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