By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra info for Advances in lipid research. Vol. 9
8 (top) and 9 (bottom). Sections of aorta labeled by incubation with choline- 3 H. The preparation in Fig. 8 was subjected to partial dehydration and e m b e d d e d in Epon. The material in Fig. 9 was dehydrated and embedded in Aquon. The radioautographic reaction, which represents labeled lecithin is concentrated over the smooth muscle cells of the aortic media, and there is little if any label over the elastic laminae. Fig. 8, X 1900; Fig. 9, X 2000. (Fig. 8 from O. Stein and Stein, 1970, reproduced by permission of the Editor of Lab.
STEIN AND Y. STEIN curs between lecithin molecules of the endoplasmic reticulum and those of mitochondria (apparently mostly of the outer mitochondrial membrane). This conclusion was reached independently from the finding of a constant ratio of grain counts over the respective organelles in sections obtained from liver pulse-labeled for 5 minutes and chased for up to 2 hours (Fig. 18) and of a rather constant ratio of specific activity of lecithin isolated from mitochondria and microsomes between 5 and 60 minutes after injection of various precursors.
Stein and Stein, 1966c, reproduced by permission of the Editor of Israel J. Med. ) 42 O. STEIN AND Y. STEIN intestine, lactating mammary gland), in the case of adipose tissue the lipid released into the circulation is free fatty acid, not triglycéride. The first electron microscopic radioautograph published was that of Sheldon and Angel (1964), who have shown concentration of silver grains over the bulk lipid of adipose tissue cells incubated with labeled palmitic acid for 3 hours. The initial steps of fatty acid transport, esterification and triglycéride deposition, were studied on parametrial adipose tissue, labeled for very short periods of time by perfusion with oleic acid- 3 H, with the help of electron microscopic radioautography (O.