Amyloid Precursor Protein: A Practical Approach by Weiming Xia, Huaxi Xu

By Weiming Xia, Huaxi Xu

Within the look for an efficient therapy for Alzheimer's ailment, APP is a distinct version protein that illustrates the big choice of simple and complex characterization innovations to be had. Exploring quite a few organic concepts to explain the constitution and serve as of this transmembrane protein, this article offers each one technique with designated, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.

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To stop the reaction, spin the beads at 3000 g for 5 min. 0) per tube. 2 M ethanolamine, then incubate in 10 ml ethanolamine for 2 hr at room temperature with rocking. 7. Centrifuge and transfer each pellet to a 2-ml Eppendorf tube. 8. 0; mix and then spin at 10,000 g for 30 sec. 0; mix and spin. Expose beads to glycine buffer for as short a time as possible. 9. 01% thimerosal for each tube. Remove 100 µl of supernatant to check later (C). To check the efficiency of conjugation, the amount of remaining IgG can be detected in the supernatant (A, B, and C) by Western blot.

Chem. 270, 4205, 1995. 13. Murayama, Y. et al. Cell surface receptor function of amyloid precursor protein that activates Ser/Thr kinases. Gerontology 42, 2, 1996. 14. Zheng, H. et al. beta-Amyloid precursor protein-deficient mice show reactive gliosis and decreased locomotor activity. Cell 81, 525, 1995. 15. Hendriks, L. and Van Broeckhoven, C. A beta A4 amyloid precursor protein gene and Alzheimer’s disease. Eur. J. Biochem. 237, 6, 1996. 16. P. Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives.

Weidemann, A. et al. Identification, biogenesis, and localization of precursors of Alzheimer’s disease A4 amyloid protein. Cell 57, 115, 1989. 3. Schubert, W. et al. Localization of Alzheimer beta A4 amyloid precursor protein at central and peripheral synaptic sites. Brain Res. 563, 184, 1991. 4. Okamoto, T. et al. Intrinsic signaling function of APP as a novel target of three V642 mutations linked to familial Alzheimer’s disease. EMBO J. 15, 3769, 1996. 5. Zambrano, N. et al. Interaction of the phosphotyrosine interaction/phosphotyrosine binding-related domains of Fe65 with wild-type and mutant Alzheimer’s beta-amyloid precursor proteins.

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