Atomic Force Microscopy: Biomedical Methods and Applications by Davide Ricci, Pier Carlo Braga

By Davide Ricci, Pier Carlo Braga

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Extra resources for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)

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The uptake of mass as a result of specifically interacting molecules is doubled in this manner, and the cantilever does not respond to temperature changes via a bimetallic effect. Additionally, the preparation involves fewer steps as in the case of the static detection mode (5). 4. Setups At the Institute of Physics at the University of Basel, Basel, Switzlerland, in collaboration with the IBM Research Laboratory Zurich, we developed cantilever array setups both for static and dynamic mode operation in liquids and in the gas phase.

1 Example of a fixed fibroblast imaged in air in the contact mode. 2. LIVE CELLS IN VITRO Live cells on untreated cover slips rarely produce acceptable contact mode images because of poor adherence to the substrate, gross fouling of the tip, and destructive tip–membrane interactions (see Notes 1–3). However, cells grown on surface-treated culture dishes offer greatly improved imaging conditions. Continuous scanning at linear scan speeds of 150 µm/s and at force loads in the low-nN range can now be conducted over several hours without any apparent damage to viable cells.

It is preferable to grow the cell culture directly in a culture dish that will then constitute the fluid cell for the Explorer Stage. Several of the AFM manufacturers now offer comparably equipped standalone instruments or equivalent optional attachments. The preferred scanner for the latter procedure should have a maximum field of view of some 130 × 130 µm2 and a z range of 10 µm. All combinations of instrumentation will accommodate the full range of relevant operational modes: contact, in situ intermittent-contact in fluid, and F-d analysis.

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