Bacteriophages: Methods and Protocols, Volume 1: Isolation, by K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton,

By K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench (auth.), Martha R.J. Clokie, Andrew M. Kropinski (eds.)

Ranging from the evolution of pathogenicity to oceanic carbon biking, the various and sundry roles that bacteriophages play in microbial ecology and evolution have encouraged elevated curiosity in the medical group. Bacteriophages: equipment and Protocols pulls jointly the mammoth physique of information and services from most sensible overseas bacteriophage researchers to supply either classical and state of the art molecular options. With its well-organized modular layout, Volume 1: Isolation, Characterization, and Interactions examines a large number of subject matters, together with the isolation of phages, morphological and molecular characterization, and interplay with micro organism. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters include short introductions to the topic, lists of the required fabrics and reagents, effortlessly reproducible laboratory protocols, and a Notes part which information pointers on troubleshooting and averting recognized pitfalls.

Thorough and state-of-the-art, Bacteriophages: tools and Protocols is a precious reference for skilled bacteriophage researchers in addition to an simply obtainable advent for beginners to the subject.

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4 Notes 1. It is recommended that careful attention should be paid to collecting the samples in such a way as not to contaminate the outside of the storage container or the person collecting 20 Van Twest and Kropinski 2. 3. 4. 5. 6. 7. 8. 9. the samples. Please note that raw sewage will most definitely contain human pathogens. For the bacteria listed above, Difco Luria broth or Tryptic Soy Broth work well. de/). Many phages require 1–10 mM divalent ions such as Ca2+ or Mg2+ for attachment or intracellular growth (18, 19, 20).

0 with HCl before the volume is topped up to 1 l (Note 1). Once sterilised by autoclaving ASW can be kept at room temperature for several months. Isolation of Cyanophages from Aquatic Environments 35 3. 86 g/l, MnCl2 . 81 g/l, ZnSO4 . 222 g/l, Na2Mo04 . 39 g/l, CuSO4 . 008 g/l, Co(N03 )2 . 0049 g/l, FeCl3 . 5 g/l. Once sterilised by filtering through a filter with a pore size of 0. 22 μm, the trace metal stock can be stored at 4◦ C for several years. 3 Water Samples Water samples that contain cyanophages will be needed to be collected in advance.

C. N. E. F. Hatfull. (1999). Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc. Natl. Acad. Sci. USA 96, 2192–2197. 7. Moreira, D. (2000). Multiple independent horizontal transfers of informational genes from bacteria to plasmids and phages: implications for the origin of bacterial replication machinery. Mol. Microbiol. 35: 1–5. 8. F. (2005). Bacteriophages and bacterial virulence, in Bacteriophages: Biology and Applications, (Kutter E. ), CRP Press, FL, pp.

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