Biophysics of DNA-Protein Interactions: From Single by Mark C. Williams, III, L. James Maher

By Mark C. Williams, III, L. James Maher

This ebook offers a concise review of present learn at the biophysics of DNA-protein interactions. quite a lot of new and classical equipment are awarded through authors investigating actual mechanisms in which proteins engage with DNA. for instance, numerous chapters handle the mechanisms through which proteins look for and realize particular binding websites on DNA, a procedure serious for mobile functionality. unmarried molecule tools corresponding to strength spectroscopy in addition to fluorescence imaging and monitoring are defined in those chapters in addition to different components of the publication that handle the dynamics of protein-DNA interactions. different vital subject matters contain the mechanisms during which proteins have interaction DNA sequences and/or modify DNA constitution. those easy yet very important version interactions are then put within the broader organic context with dialogue of bigger protein-DNA complexes . themes comprise replication forks, recombination complexes, DNA fix interactions, and finally, easy methods to comprehend the chromatin context of the mobile nucleus. This publication might be of curiosity to readers who desire to discover present biophysical techniques to DNA-protein interactions throughout a number of degrees of organic complexity.

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Additional resources for Biophysics of DNA-Protein Interactions: From Single Molecules to Biological Systems

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This theoretical estimation awaits experimental validation described in Sect. 2, where the DNA longitudinal fluctuations will be investigated through SD measurements of single specifically bound proteins. 6 Prospects for LacI Sliding Studies Using SMID More experiments and analysis are necessary to unravel the sliding mechanisms of proteins on DNA using SMID. Here, we lay out necessary investigations in detail. We first describe three different, but complementary approaches that should be used in the study: analytical calculations, simulations, and experimental measurements.

2. The Escherichia coli repressor-operator interaction: equilibrium measurements. Biochemistry 20:6948–6960 9. Jack WE, Terry BJ, Modrich P (1982) Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence. Proc Natl Acad Sci U S A 79:4010–4014 10. Ricchetti M, Metzger W, Heumann H (1988) One-dimensional diffusion of Escherichia coli DNA-dependent RNA polymerase: A mechanism to facilitate promoter location. Proc Natl Acad Sci U S A 85:4610–4614 11.

Science 268:83–87 33. Kalodimos CG, Biris N, Bonvin AMJJ, Levandoski MM, Guennuegues M, Boelens R, Kaptein R (2004) Adaptation in nonspecific and specific protein-DNA complexes. Science 305:386–389 34. Smith SB, Finzi L, Bustamante C (1992) Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads. Science 258:1122–1126 35. Thompson RE, Larson DR, Webb WW (2002) Precise nanometer localization analysis for individual fluorescent probes. Biophys J 82:2775–2783 36.

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