Cell Biology and Histology by Leslie P. Gartner PhD, James L. Hiatt PhD, Judy M. Strum PhD

By Leslie P. Gartner PhD, James L. Hiatt PhD, Judy M. Strum PhD

Univ. of Maryland, Baltimore. examination evaluate of mobile biology and heritage for college kids learning for the USMLE Step 1 and for path tests. define layout. Softcover. prior version: c1993. DNLM: Histology--outlines.

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11. Survey of Glycosyltransferases by Type of Sugar Transferred A. SIALYLTRANSFERASES I. Number and Types of Siulyltrunsferases The immediate precursor of glycosidically bound sialic acid is the sugar nucleotide, CMP-sialic acid. With this donor substrate, sialyltransferases catalyze the formation of sialic acid glycosides by transglycosylation according to reaction 2: CMP-sialic acid t HO-acceptor+Sialyl-0-acceptor + CMP-H (2) As shown in Table I, five sialic acid linkages commonly occur in a wide variety of mammalian glycoconjugates--Siaa2+6Gal, Siaa2BGa1, Siacu2-t 6GalNAc, Siaa2+6GlcNAc, and Siaa24Sia-and there is a single recent report of a Siaa2+4Gal linkage (36) and of a Siaa2+4GlcNAc linkage (20).

3 I Siaa2,6Gal81,4GlcNAcBi, 2 M a n a I,3 \ 0 ,Mond1,4GlcNAcBl,4 \ \ GalBI,4 2 Manul, 6 ‘GlcNAcBI, Fucal,3 / F u c u I,6 0 0 II 0 Siau2,3GalB1,3 @ /GlcNAcB‘sAsn ‘~alNAca-O-Thr/Ser @ Siau 2,s’~ III Fucal,2 GolNAcul,3 @ @ ,GOlBI,3 \ @ \GalNAca-O-Thr/Ser Siau2,6’@ Ip P ~ G ~ c U A B ~ , ~ G ~ ~ N A C ~ S B ~ , ~ ) ~ - G ~ C U,A 4 XBy ~l B, I ~- OG- ~Ser ~ B I , ~ G ~ ~ B I PT G l c u l , 2 G a l S I - O - Hyl @ 26 @ @ O 27 G L Y COSY LTRANSFERASES 2. The actions of different glycosyltransferases in oligosaccharide biosynthesis.

Purification of Glycosyltransferases A. General Considerations B. Affinity Chromatographic Methods C. Stabilization of Glycosyltransferases during Purification and Storage D. Criteria of Purity VI. Concluding Remarks Acknowledgment References 97 108 113 113 113 116 121 122 126 132 133 134 138 141 144 144 145 154 156 157 158 158 1. Introduction The glycosyltransferases comprise a group of enzymes that catalyze the synthesis of specific glycosides by transfer of a monosaccharide from a glycosylnucleotide (nucleotide-sugar) donor substrate to an acceptor substrate as shown in reaction 1: Glycosylnucleotide t acceptor +.

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