Current Protocols in Molecular Biology (1988 - 2003) by Frederick M. Ausubel (Editor), David Moore

By Frederick M. Ausubel (Editor), David Moore (Editor), David D. Moore (Editor), Roger Brent (Editor), J. G. Seidman (Editor)

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On this medium, these vector-containing cells possess β-galactosidase activity and turn blue. Lactose Analogs There are many compounds related to lactose that were first used for the biochemical and genetic analysis of lac operon activity and are Contributed by Elisabeth A. 14 Copyright © 2002 by John Wiley & Sons, Inc. 1 Stock conc. (mg/ml) Antibioticb Ampicillinc Final conc. 1 M sodium phosphate buffer, pH 8 Nalidixic acid, pH to 11 with NaOH Rifampicin,e in methanol Mode of action Mode of resistance Bacteriocidal; only kills growing E.

Many vectors incorporate a lac α-fragment gene, which is small and easily manipulated. Exploitation of these vectors requires use of a strain carrying the complementing ω-fragment gene to allow assembly of an active complex. This gene is often carried on an F′ plasmid (see below). 2). On this medium, these vector-containing cells possess β-galactosidase activity and turn blue. Lactose Analogs There are many compounds related to lactose that were first used for the biochemical and genetic analysis of lac operon activity and are Contributed by Elisabeth A.

Omega fragment protein containing the carboxy-terminal fragment of β-galactosidase. This protein lacks enzymatic activity, but βgalactosidase activity can be restored when the peptide is complexed with an alpha fragment. ori (origin) site on genome at which DNA replication begins. outgrowth the growth of freshly transformed cells under nonselective conditions for enough time to allow proteins encoded by the foreign DNA to be expressed. overnight a small, freshly saturated liquid culture of bacteria.

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