DNA Binders and Related Subjects: -/- by Peter B. Dervan, Adam T. Poulin-Kerstien (auth.), Michael J.

By Peter B. Dervan, Adam T. Poulin-Kerstien (auth.), Michael J. Waring, Jonathan B. Chaires (eds.)

The editors have assembled an eclectic number of articles that provide an excellent review of the present cutting-edge in small molecule-nucleic acid targeting.......

As indicated by means of its basic identify, this newest contribution to the Topics in present Chemistry sequence isn't intended to be finished. quite, a number of issues on the vanguard of small molecule-nucleic acid biochemistry are mentioned in nice aspect. This quantity may be of substantial use either to researchers within the box and to those that desire to movement into this intriguing area.

Paul J. Hergenrother, college of Illinois at Urbana-Champaign, JA059748D, J.AM.CHEM.SOC., Vol. 128, no. 10, 2006

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It should be emphasized that the array of structures listed in Table 1 are but a point of departure. The competition dialysis method is completely general, and arrays of structures of particular interest can be designed as desired. The only limitations are that the structures be large enough to be retained by the dialysis tubing chosen for use, and that they are verified to be stable under the ionic conditions of the experiment. Essential quality control experiments to characterize nucleic acid samples were described in detail [41, 42] and include UV absorbance and circular dichroism spectroscopy, and thermal denaturation studies.

Fig. 1 Schematic of the competition dialysis experiment. A schematic representation of the structures used is shown on the left. The center shows the simple experimental setup, a beaker containing the test ligand solution and the holder for the dialysis units. A schematic of the microdialysis unit is shown on the right Structural Selectivity of Drug-Nucleic Acid Interactions 37 The competition dialysis assay described here [32] is a simple and straightforward extension of Crothers’ original method.

35 35 37 38 39 41 3 Global Analysis of the Data from the First Generation Assay . . . . . . 1 Trends from Tukey Box Plots . . . . . . . . . . . . . . 2 New Metrics: the Specificity Sum (SS) and SS/Cmax . . . . . . . . 42 42 45 4 Quantitative Analysis of Competition Dialysis Data: Validation . . . . . 49 5 Summary and Outlook . . . . . . . . . . . . . . . . 50 . . . . . . . . . . . . . . . . . . . . 51 References . . . . . .

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