Field-Flow Fractionation in Biopolymer Analysis by Karl-Gustav Wahlund, Lars Nilsson (auth.), S. Kim R.

By Karl-Gustav Wahlund, Lars Nilsson (auth.), S. Kim R. Williams, Karin D. Caldwell (eds.)

This is a well timed number of very important biomedical purposes for a collection of separation/characterization thoughts which are swiftly becoming more popular because of their vast dynamic variety, excessive solution, and skill to operate in most typically used solvent structures. Importantly, the field-flow fractionation (FFF) process has lately emerged as a favourite supplement to dimension exclusion chromatography for protein prescribed drugs. Fractionation with FFF is mild and preserves protein structural integrity higher than current choices. within the current textual content, diverse chapters are written via specialists of their respective box of program, who provide comparisons among the FFF options and different equipment for characterizing their exact concentration fabric. useful guide-lines for winning implementation, comparable to collection of working stipulations, are provided along with each one program. as well as new instrumentation and techniques that tackle very important present subject matters, readers are supplied with an total experience of past (but undying) significant advancements which may be missed in literature searches.

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Jonsson JA, Carlshaf A (1989) Flow field-flow fractionation in hollow cylindrical fibers. Anal Chem 61(1):11–18 22. Carlshaf A, Jonsson JA (1991) Effects of ionic-strength of eluent on retention behavior and on the peak broadening process in hollow fiber flow field-flow fractionation. J Microcolumn Sep 3(5): 411–416 23. Carlshaf A, Jonsson JA (1993) Properties of hollow fibers used for flow field-flow fractionation. Sep Sci Technol 28(4):1031–1042 24. Lee WJ, Min BR, Moon MH (1999) Improvement in particle separation by hollow fiber flow field-flow fractionation and the potential use in obtaining particle site distribution.

Marcel Dekker, New York, pp 1–25 23. Belfort G, Zydney AL (1998) Chapter 15. In: Malmsten M (ed) Biopolymers at interfaces, vol 75, Surfactant science series. Marcel Dekker, New York, pp 513–559 24. Amanda A, Mallapragada SK (2001) Comparison of protein fouling on heat-treated poly(vinyl alcohol), poly(ether sulfone) and regenerated cellulose membranes using diffuse reflectance infrared Fourier transform spectroscopy. Biotechnol Prog 17:917–923 25. Koehler JA, Ulbricht M, Belfort G (2000) Intermolecular forces between a protein and a hydrophilic modified polysulfone film with relevance to filtration.

J Membr Sci 209:93–106 2 Assessing Protein-Ultrafiltration Membrane Interactions 35 17. Williams SKR, Giddings JC (2000) Sample recovery. In: Schimpf M, Caldwell K, Giddings JC (eds) FFF handbook. Wiley, New York 18. Zeman LJ, Zydney AL (1996) Microfiltration and ultrafiltration principles and applications. Marcel Dekker, Inc, New York 19. Matthiasson E (1983) The role of macromolecular adsorption in fouling of ultrafiltration membranes. J Membr Sci 16:23–36 20. Lyklema J, Norde W (1996) Interfacial behaviour of biomacromolecules.

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