Foreign Gene Expression in Fission Yeast: by Yuko Giga-Hama (auth.), Yuko Giga-Hama Ph.D., Hiromichi

By Yuko Giga-Hama (auth.), Yuko Giga-Hama Ph.D., Hiromichi Kumagai Ph.D. (eds.)

Recombinant proteins, produced in cells or microorganisms, play an enormous function in uncomplicated examine, scientific technological know-how and the biotechnology undefined. Many international gene expression structures utilizing mammalian cells, E. coli, insect cells, yeast and different organisms as hosts were built. in spite of the fact that, only in the near past has the expression procedure of Schizosaccharomyces pombe as a number turn into a subject matter of concentration, because it is predicted to be ecocnomic for expressing international genes. this is because S. pombe is evolutionarily extra complex than different forms of yeast. This booklet describes using S. pombe in uncomplicated study and business applications.

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Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Plasmid 1986; 15:156-158. Foreign Gene Expression in Fission Yeast Schizo saccharomyces pombe 38 10. Picard D, Schena M, Yamamoto KR. An inducible expression vector for both fission and budding yeast. Gene 1990; 86:257-261. 11. 12. 13. 14. 15. 16. 17. Russel P. Gene cloning and expression in fission yeast. In: Nasim A, Young P, Johnson BF, eds. Molecular Biology of the Fission Yeast.

Cell 1988; 53:659-667. 59. Toyama R, Okayama H. Human chorionic gonadotropin a and human cytomegalovirus promoters are extremely active in the fission yeast S. pombe. FEBS Lett 1990; 268:217-221. 60. Toyama R, Bende SM, Dhar R. Transcriptional activity of the human immunodeficiency virus-l LTR promoter in fission yeast Schizosaccharomyces pombe. Nucleic Acid Res 1992; 20:2591-2596. 61. Swaminathan S, Malhotra P, Manohar CF et al. Activation of a dual adenovirus promoter containing non consensus TAT A motifs in S.

40. Russell PR. Transcription of the triose-phosphate isomerase gene of Schizosaccharomyces pombe initiates from a start point different from that in Saccharomyces cerevisiae. Nature 1983; 3°1:167-169. 41. Kuranda MJ, Robbins PW. Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae. Proc Nat! Acad Sci USA 1987; 84:2585-2589. 42. Jones R, Moreno S, Nurse P et al. Expression of the SV 40 promoter in fission yeast: identification and characterization of an API-like factor.

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