Hepatocytes: Methods and Protocols by Christiane Guguen-Guillouzo, Andre Guillouzo (auth.),

By Christiane Guguen-Guillouzo, Andre Guillouzo (auth.), Patrick Maurel (eds.)

Hepatocytes account for roughly eighty% of the liver mass and play an important function in a variety of points of liver physiopathology, displaying unequalled complexity and variety of features. In Hepatocytes: equipment and Protocols, professional researchers give you the reader with tools, technical protocols, and evaluate chapters concentrating on chosen parts of hepatocyte biology together with isolation, tradition, differentiation and stem cells, and hepatocyte use in scientific, easy, and utilized learn. With a selected emphasis on human hepatocytes, the amount offers chapters overlaying matters together with hepatocyte tradition versions, cryopreservation equipment, differentiation review, liver ontogenesis, construction of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and shipping, bile acid and blood coagulation issue construction, an infection via HBV and HCV, humanized animals, biortificial liver units, hepatocyte transplantation. As a quantity within the hugely profitable Methods in Molecular Biology™ sequence, protocol chapters contain short introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and keeping off recognized pitfalls.

Comprehensive and state of the art, Hepatocytes: tools and Protocols could be important to all those who find themselves at the moment utilizing or making plans to exploit human, or animal, hepatocytes to enquire any point of liver physiopathology or who're attracted to liver improvement or liver stem cells and liver biotherapy.

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Accordingly, after phenobarbital (PB) treatment it was found that hierarchical clustering of the human hepatocytes but not human hepatoma cell lines shifted from donor specific to treatment specific when the probe sets were filtered to focus on PB-induced genes. Comparison of various human hepatocyte populations at the whole genome level has evidenced that the magnitude of conserved gene expression changes among donors is very small with fewer than 50% of the gene responses Hepatocyte Models and Applications 21 altered by a chemical in more than one donor.

Conclusions Considerable progress has been made over the last 40 years in the understanding of the biology of the hepatocyte and liver diseases caused by xenobiotics or biological agents (viruses and parasites) as well as in hepatocyte-based therapies by using suspended and cultured hepatocytes. Primary human hepatocytes remain the most pertinent in vitro liver model, being the closest to the liver in vivo; however, they have serious drawbacks, due to their low access, large donor-to-donor functional variability, and early phenotypic changes occurring in culture.

Guguen-Guillouzo, C. and Guillouzo, A. (1983) Modulation of functional activities in cultured rat hepatocytes. Mol. Cell Biochem. 53–54, 35–56. 10. Guillouzo, A. (1998) Liver cell models in in vitro toxicology. Environ. Health Perspect. 106 Suppl 2, 511–532. 11. Guillouzo, A. and Guguen-Guillouzo, C. (2008) Evolving concepts in liver tissue modeling and implications for in vitro toxicology. Expert Opin. Drug Metab. Toxicol. 4, 1279–1294. 12. , Kenna, 13. 14. 15. 16. 17. 18. 19. 20. G. (2007) Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies.

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