By Sarah A. Schwartz, Richard M. Caprioli (auth.), Stanislav S. Rubakhin, Jonathan V. Sweedler (eds.)
Mass spectrometry (MS) deals unequalled features for the detection, characterization, and id of a extensive diversity of analytes. Mass spectrometry imaging (MSI) integrates MS info with details at the spatial distributions of the analytes, additional improving the applicability of MS. In Mass Spectrometry Imaging: ideas and Protocols, professional practitioners from academia, undefined, and the medical institution give a contribution state of the art protocols describing the applying of MSI to investigations of analyte localization in various specimens, from microorganisms to plant and animal tissues. Divided into 3 sections, this quantity provides the rules of MS, present and destiny tendencies of MSI, and qualitative and quantitative protocols to degree and determine endogenous metabolites and xenobiotics. An array of MSI techniques and applied sciences for characterizing peptide and protein distributions are defined intimately. Written within the hugely profitable equipment in Molecular Biology™ sequence layout, protocol chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, and step by step, comfortably reproducible laboratory techniques. additionally integrated are notes offering how one can stay away from experimental pitfalls and necessary feedback for approach troubleshooting. accomplished and up to date, Mass Spectrometry Imaging: ideas and Protocols is written for scientists, organic and chemical engineers, and clinicians who're attracted to utilising MSI of their paintings and those that would receive advantages from having particular experimental guidance to be had in one, handy source.
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Additional info for Mass Spectrometry Imaging: Principles and Protocols
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These collisions result in a change in ion motion trajectory and/or velocity and consequently, distortion of signal or even ion recombination and loss of signal. Although such effects can be negative, they can also be beneficial, depending on whether the ions need to be kinetically cooled or to achieve 34 Rubakhin and Sweedler controlled fragmentation. Typically, fragmentation is performed at selected locations in the instrument by adding a collision gas into the ion flight path collision cell or directly into the mass analyzer.