Mass Spectrometry Imaging: Principles and Protocols by Sarah A. Schwartz, Richard M. Caprioli (auth.), Stanislav S.

By Sarah A. Schwartz, Richard M. Caprioli (auth.), Stanislav S. Rubakhin, Jonathan V. Sweedler (eds.)

Mass spectrometry (MS) deals unequalled features for the detection, characterization, and id of a extensive diversity of analytes. Mass spectrometry imaging (MSI) integrates MS info with details at the spatial distributions of the analytes, additional improving the applicability of MS. In Mass Spectrometry Imaging: ideas and Protocols, professional practitioners from academia, undefined, and the medical institution give a contribution state of the art protocols describing the applying of MSI to investigations of analyte localization in various specimens, from microorganisms to plant and animal tissues. Divided into 3 sections, this quantity provides the rules of MS, present and destiny tendencies of MSI, and qualitative and quantitative protocols to degree and determine endogenous metabolites and xenobiotics. An array of MSI techniques and applied sciences for characterizing peptide and protein distributions are defined intimately. Written within the hugely profitable equipment in Molecular Biology™ sequence layout, protocol chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, and step by step, comfortably reproducible laboratory techniques. additionally integrated are notes offering how one can stay away from experimental pitfalls and necessary feedback for approach troubleshooting. accomplished and up to date, Mass Spectrometry Imaging: ideas and Protocols is written for scientists, organic and chemical engineers, and clinicians who're attracted to utilising MSI of their paintings and those that would receive advantages from having particular experimental guidance to be had in one, handy source.

Show description

Read or Download Mass Spectrometry Imaging: Principles and Protocols PDF

Best biology books

Receptor Binding Techniques

Retaining the excessive criteria set by way of the winning first variation, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical and academia make the most of the newest advancements to supply certain sensible equipment for learning receptors in silico, in vitro, and in vivo.

Angiogenesis: In Vitro Systems Part A

Angiogenesis is the expansion of recent blood vessels and is a vital ordinary technique within the physique. A fit physique continues an ideal stability of angiogenesis modulators. in lots of critical ailment states, even if, the physique loses regulate over antiogenesis. illnesses which are angiogensis-dependent end result whilst blood vessels both develop excessively or insufficiently.

Systems Biology and Regulatory Genomics: Joint Annual RECOMB 2005 Satellite Workshops on Systems Biology and on Regulatory Genomics, San Diego, CA, USA; December 2-4, 2005, Revised Selected Papers

This booklet constitutes the completely refereed post-proceedings of 2 joint RECOMB 2005 satellite tv for pc occasions: the 1st Annual Workshop on platforms Biology, RSB 2005 and the second one Annual Workshop on Regulatory Genomics, RRG 2005, held in San Diego, CA, united states in December 2005. The 21 revised complete papers awarded have been conscientiously reviewed and chosen for inclusion within the booklet.

Additional info for Mass Spectrometry Imaging: Principles and Protocols

Example text

Wiseman, J. , Ifa, D. , Kissinger, C. , Manicke, N. , Kissinger, P. , Cooks, R. G. (2008) Desorption electrospray ionization mass spectrometry: Imaging drugs and metabolites in tissues. Proc Natl Acad Sci U S A, 105, 18120–18125. 68. , Vertes, A. (2007) Atmospheric pressure molecular imaging by infrared MALDI mass spectrometry. Anal Chem, 79, 523–532. 69. , Barton, A. , Vertes, A. (2008) Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry.

Marshall, P. , Princivalle, A. , Clench, M. R. (2008) Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. Anal Chem, 80, 8628–8634. 61. Luxembourg, S. , Mize, T. , McDonnell, L. , Heeren, R. M. (2004) High-spatial resolution mass spectrometric imaging of peptide and protein distributions on a surface. Anal Chem, 76, 5339–5344. 62. Wiseman, J. , Ifa, D. , Cooks, R. G. (2008) Ambient molecular imaging by desorption electrospray ionization mass spectrometry.

These collisions result in a change in ion motion trajectory and/or velocity and consequently, distortion of signal or even ion recombination and loss of signal. Although such effects can be negative, they can also be beneficial, depending on whether the ions need to be kinetically cooled or to achieve 34 Rubakhin and Sweedler controlled fragmentation. Typically, fragmentation is performed at selected locations in the instrument by adding a collision gas into the ion flight path collision cell or directly into the mass analyzer.

Download PDF sample

Rated 4.02 of 5 – based on 39 votes