By David M. Neville Jr. (auth.), Edward D. Korn (eds.)
Volume three maintains the strategy performed within the first volumes of this se ries of publishing articles on membrane method which come with, as well as procedural information, incisive discussions of the ap plications of the equipment and in their barriers. Wh at is the theoretical foundation of the strategy, how and to what difficulties can it's utilized, how does one interpret the consequences, what has to date been completed by means of the strategy, what lies within the future-these are the questions the authors have attempted to respond to. No region of membrane biology engages the curiosity of extra investigators than reports of the plasma membrane. 4 chapters during this quantity are eager about a number of features of the mobile floor. primary to all reports of the mobile floor are the isolation and characterization of natural plasma membranes. Many arrangements defined within the literature are insufficient or are inadequately characterised. within the first bankruptcy, Neville discusses the theoretical and functional bases of tissue fractionation, empha sizes the differences in enzyme content material between plasma membranes from diversified assets, bargains information within the number of the correct standards for assessing membrane purity, and indicates the easiest markers for detecting the prospective presence of contaminating organelles. to study intimately all of the many arrangements of plasma membranes which were released is impossible.
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Extra resources for Methods in Membrane Biology: Volume 3 Plasma Membranes
And Hare, J. , 1973, A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system, Biochirn. Biophys. Acta 311:173-179. Levitan, 1. , Mushynski, W. , 1972, Highly purified synaptosomal membranes from rat brain: Preparation and characterization, J. Biol. Chern. 247: 5376-5381. , 1973, The plasma membranes of bovine circumvallate papillae: Isolation and partial characterization, Biochirn. Biophys. Acta 291:650-661. , 1970, A new method for the isolation of plasma membranes from pig Iiver, Hoppe-Seylers Z.
Since this organelle contributes less than 25% of the homogenate protein in liver (an ER-rich tissue by electron microscopy) a fourfold marker enrichment in a microsomal fraction would appear to be a minimum requirement for a specific marker. That this degree of purification is often not observed may reflect the presence of smooth membranes of nonER origin in the microsomal fraction or the localization of the markers in membranes other than the ER. Total quantitative fractionation is the best approach to validating ER markers.
6. EXPLANATION OF TABLE I The literature survey on which Table I is based extends from 1960 to late 1973. Only surface membranes derived from warm-blooded vertebrates are inc1uded. Red blood cell membranes and myelin have not been inc1uded. A number of intestinal brush border preparations, each differing slightly in methodology, are omitted since these have been covered in a recent review (Porteous, 1972). Table I is presented in outline form subheaded with organ and species of origin along with a reference in the first column.