Molecular Motors: Methods and Protocols (Methods in by Ann O. Sperry

By Ann O. Sperry

Molecular motor proteins produce strength for stream in an exceptionally good selection of mobile procedures. This quantity explores the extraordinary practical and structural range of molecular vehicles and provides tools appropriate to every motor relations. additionally, it describes ideas directed at cars that fall outdoor of the 3 characterised households: dynamin and F1ATPase.

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Extra resources for Molecular Motors: Methods and Protocols (Methods in Molecular Biology Vol 392)

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Depending on the type of centrifuge tubes used, it may be necessary to machine a small fitting for mounting the coverslips securely at the bottom of the tube. It is important that the coverslip fits securely into the tube to ensure all microtubules are pelleted onto the glass. We routinely spin microtubules onto 5-mm-diameter coverslips in 8-mm (outer diameter) centrifuge tubes using a Beckman airfuge. As an alternative to a machined fitting for the centrifuge tubes, a small amount of epoxy can be placed in the bottom of the tube and then spun while the epoxy is curing; this will generate a flat-angled surface upon which to lay the coverslip.

138, 1711–1722. N. (1998) Characterization of microtubule 7. binding domains in the Arabidopsis kinesin-like calmodulin-binding protein. Plant Cell 10, 957–965. S. (2004) KIC, a novel Ca2+ 8. binding protein with one EF-hand motif, interacts with a microtubule motor protein and regulates trichome morphogenesis. Plant Cell 16, 185–200. N. (2002) The calmodulin-binding domain from a 9. plant kinesin functions as a modular domain in conferring Ca2+-CaM regulation to animal plus- and minus-end kinesins.

4. 2 mL deionized water, and degas this solution for 10 min. Then add 100 µl 10-ammonium persulfate and 20 µL TEMED, and mix the solution by swirling gently (see Note 18). 5. Pour off the isobutyl alcohol and rinse the top of the separating gel with water to completely remove any residual isobutyl alcohol. 6. Using a Pasteur pipet, pour the stacking solution and insert 1-mm-thick combination into the stacking solution. Allow stacking solution to polymerize for about 30 min. 7. Prepare 1X electrode buffer by diluting 100 mL 5X buffer with 400 mL deionized water.

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