Physicochemical Characteristics of Oligonucleotides and by Borek Janik

By Borek Janik

Physicochemical reports on po1ynuc1eotides and their parts are a comparatively new box which, virtually day-by-day, draws an ever­ expanding variety of scientists. to this point, despite the fact that, just a restricted attempt has been made to bring together the enormous quantity of physicochemical facts to be had right into a important layout. I initially undertook this compilation of information to complement my very own study efforts. however it quickly grew to become glaring that my labors could be of profit to different staff during this advanced quarter, and this quantity is the outcome. the data stated during this manuscript covers the literature as much as the top of 1970. the current compilation can't most likely checklist all recorded dissociation constants, extinction coefficients, and Tm values of po1ynuc1eotides, oligonucleotides, and their com­ plexes. As defined within the "Explanation of Tables," a few discre­ tion needed to be exercised within the number of oligonucleotides and po1ynuc1eotides for inclusion. each attempt has been made, besides the fact that, to supply greater than only a consultant move component to the on hand literature.

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1 !! phosp. 260* 11. 9)% 28 7 260* 11. 02 !! 01!! 1 !! 0 (11. h. 1 !! 1!! 1 !! 05 !! phosp. 05 !! phosp. 1 !! 20 4 4 112 m 4 2. 14 Ref. h. 5 260* 1 258 11. 5 28 7 260* 11. 3(11. 1 ~ phosp. 5 260 11. h. 1. 1. 0 A. 03 ~ phosp. 05 ~ phosp. 3(260) NaC1 Ref. lMKOH 261 31. 1 M KOH 260* 31. 4 7 O. 05 ~ phosp. 05 ~ phosp. l1 m 11. h. 89)n 284 60 01igomers C-C-Gp 2. SPECTRAL DATA Medium pH A mJ.! 1 l:! 1 l:! 05 l:! phosp. 05 l:! phosp. 1 l:! 5(260) 24 4 112 4 4 ll2 250 (255, 284 280),1. 20 (1. h. 02 l:!

3. 4. Po1~ A pKpolym Note Ref. :! :! :! :! :! :! :! :! :! :! :! 00 279 1. DISSOCIATION PROPERTIES 26 Complex Po1~ Method T °c Medium pKpo1ym Note Ref. 1 !! 01!! acet. 1 !! 01!! acet. 1 !! 01!! acet. 1 !! 15 !! 2 !! 3 !! 4 !! 6 !! 8 !! 49 A·2U - A·A+U 41 S 20 1 !! 35 A·2U- A·A+U 41 260 22 acet. (< pH4), form. 01!! 1 !! bor. 2 co. ,rev. 1!! 05 !! 15 !! 15 !! 01!! ~g 10 !! 3. POLYNUCLEOTIDES Complex Method T °c Medium PKpolym Note Ref. 15 !! 01 !! 15 !! 15 !! 15 !! 22 ;11. 05 co. 16 co. 15!! ' Poly dA ComElexes 2 Poly U 260 22 acet.

8 h Note Ref. h. h. 1. OLIGONUCLEOTIDES 01igomers U-C (Con't) Medium pH A mfJ. E x 10- 3 1/mo1·cm h % Note Ref. 1 !! 7!! Ol!! 1 !! 6 260* dU-G 2 258 U-Gp 1 O. I!! 05 !! phosp. 05 !! phosp. 1 !! 7 !! Ol!! h. 28)n 55 A-A-C 1 259 11. 3 (11. 5 28 56 01igomers 2. 0 Ref. 1 phosp. h. 2 ! 05 ~ phosp. 05 ~ phosp. h. 1. 0 (1. 6 h Ref. 1 !! phosp. 260* 11. 9)% 28 7 260* 11. 02 !! 01!! 1 !! 0 (11. h. 1 !! 1!! 1 !! 05 !! phosp. 05 !! phosp. 1 !! 20 4 4 112 m 4 2. 14 Ref. h. 5 260* 1 258 11. 5 28 7 260* 11. 3(11.

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