Pulsed-Field Gel Electrophoresis: Protocols, Methods, and by George F. Carle, Georges F. Carle (auth.), Margit

By George F. Carle, Georges F. Carle (auth.), Margit Burmeister, Levy Ulanovsky (eds.)

...an first-class reference and experimental manual...a needs to for each biochemistry and molecular biology library.-Journal of the yank Chemical Society

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3. Pour the molten agarose into the mold and allow it to soliclify. 4. Carefully remove the comb and disassemble the gel mold (sapNote 12). 5. Cut the sample blocks to slightly less than the dimensions of the wells. Place the agarose plug from which the sample is to be cut on a clean glass slide. Lay the comb flat and at one edge of the slide as a guide (seeNote 13). With a spatula in one hand, gently pin the plug against the comb while cutting with a surgical scalpel held in the other hand (seeNote 14).

8 V/cm) for 24 h (not shown). Acknowledgments The methods development and the results presented were largely done at the University of California at Davis with the support of NIH and DOE grants to Dr. E. Morton Bradbury. This work at the City of Hope is supported by an NIH grant to Dr. Arthur D. Riggs. I thank Ronald Goto and Dr. Marcia Miller at the City of Hope for use of and help with their CHEF, which is supported with NIH Biomedical Research Support Grant#S07RRO54’71. I thank Jodie Galbraith of U.

When referring to publishecl tables to choose the correct condition for the size range of interest, be aware that temperature, voltage, and agarose concentrations should be similar in order to get comparable results. The dependence of the mol wt from the distance migrated is not simple in OFAGE (13). A s in CHEF (see Chapter 2), there are basically three zones of separation, from bottom to top: a first, main region of resolution, a region of higher resolution (by about a factor of two), and the limiting mobility zone.

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