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The looks of one other quantity in that very good sequence, equipment in Enzymology, is often a reason for appreciation in the event you desire to effectively perform a specific process or arrange an enzyme or metabolic intermediate with out the tiresome prospect of looking through unusual literature and maybe making a choice on an unproven procedure which isn't simply reproduced.
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Extra resources for Recombinant DNA Part A
This reaction, called blunt-end joining, is a powerful method for structuring DNA molecules in vitro (see below) but proceeds much less readily than nick sealing. Blunt-end joining is not linearly dependent on enzyme concentration but increases greatly at higher enzyme levels. Although T4 RNA ligase does not catalyze blunt-end joining, it markedly stimulates the reaction, particularly at low DNA ligase concentrations. 41 As much as a 20-fold increase in the rate was observed at low DNA ligase concentrations, and the stimulation was specific for blunt-end joining because RNA ligase had only a marginal influence on the joining of cohesive ends.
Biol. Chem. 244, 594 (1969). ~2W. R6wekampand R. Firtel, unpublishedresults. 48 ENZYMES IN RECOMBINANT DNA RESEARCH  Pstl CTGCAG Vector G A C G T C Vector T PstI Pstl Cleavage G 3 ' A C G T C Vector Vector C T G C A 3 ' G I Poly (dG) addition G Vector 3'GGGGGGGGACGTC Vector C T G C A G G G G G G G G 3 ' G + Insert C C C C C C C C 3 ' 3'CCCCCCCC - - ~Annealing Vector CTGCAGGGGGGGG ~ G 3'CCCCCCCC - - CCCCCCCCY G Vector GGGGGGGGACGTC Cloning PstI I Pstl CTGCAGGGGGGGG ~ CCCCCCCCTGCAG Vector msert GGGGGGGGACGTC Vector G A C G T C C C C C C C C Psd Pstl Hybrid DNA SCHEME 1 phate and to the presence of other contaminating nucleotide species.
The latter methods are easily reversible, and Ohtsuka et al. s6 used this approach to synthesize a heptadecanucleotide corresponding to bases 61-77 of E. coli tRNA fiet. The smallest monoaddition donors are the mononucleoside 3',5'-bisphosphates pCp, pAp, pUp, pGp, pdCp, pdAp, pdTp, pdUp, and pdGp. They add to the 3'-terminus of a number of different acceptors including tRNA, mRNA, and double- and singlestranded viral R N A Y ,ss These studies are examples of the important application of RNA ligase technology to the precise engineering of naturally derived RNA molecules.