By Sherry A. Dunbar, James W. Jacobson (auth.), Heide Schatten, Abraham Eisenstark (eds.)
Salmonella: equipment and Protocols provides unique tools on various features of Salmonella examine, concentrating on these which offer landmarks for destiny discovery. Highlighted subject matters comprise molecular genotyping, fast and exact new equipment for delicate selection of antibiotic resistance profiles, the characterization of Salmonella-specific mobile antigens, and Salmonella-host telephone interactions. unique tools for learning Salmonella motility also are offered. every one bankruptcy offers a quick evaluate of the subject, that is by means of precise motives of options comparable to genetic manipulation and molecular imaging that provide the probabilities of utilizing Salmonella as a healing vector to intrude with illness. this is often the 1st finished quantity of equipment and protocols for learning Salmonella and should be integral to researchers engaged within the learn of Salmonella, and enterobacteria regularly. simply because no fresh complete literature of this layout is offered on Salmonella, Salmonella: tools and Protocols can be of worth to a large choice of researchers engaged within the learn of Salmonella, and enterobacteria in general.
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Extra info for Salmonella: Methods and Protocols
Clifton, S. , et al. (2004) Comparison 5. of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid. Nat. Genet. 36, 1268–1274. 6 Chiu, C. , et al. (2005) The genome sequence of Salmonella 6. enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen. Nucleic Acids Res. 33, 1690–1698. 7 Liu, S. L. and Sanderson, K. E. (1992) A physical map of the Salmonella 7. typhimurium LT2 genome made by using XbaI analysis. J.
Externally supplied aldehyde rapidly enters the cells by diffusion. In luminescent bacteria, the long-chain aldehyde has 14 carbons, but dodecyl aldehyde is more soluble in water and hence superior as an addition. 1. To a 1-mL sample of growing bacteria in LB, add 109 –1010 F01-lux. Use a glass scintillation vial. 2. Incubate for 30 min at 28 C. 3. 1 % v/v in ethanol) and mix. To liquefy pure aldehyde, melt it in a 60 C incubator or place the bottle in water and heat. 4. After 4 min, read the light emitted in a Packard Tri-Carb (1600 TR) scintillation counter with the coincidence off.
After autoclaving, add 20 mL 10 % SDS and adjust the volume to 1 L with sterile water. N -Laurylsarcosine sodium is toxic, so wear mask when weighing. 5 M EDTA, ca. 600 mL H2 O. After autoclaving, adjust the volume to 1 L with sterile water. 5 M EDTA, ca. 700 mL H2 O, and 10 g N -laurylsarcosine sodium. After autoclaving, add 20 mL 10 % SDS and adjust the volume to 1 L with sterile water. N -Lauryl-sarcosine sodium is toxic, so wear mask when weighing. Powder of proteinase K is added to proteinase K solution to make the required concentration, which is usually 1 mg/mL, prior to use.