Somaclonal Variation in Crop Improvement I by Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

By Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

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1986)and sodium chloride (Wong et al. 1986). male sterile plants (Ling et al. 1987) high protein (Schaeffer et al. 1984) were selected. Recently. Ogura et al. (1987. S. Bajaj 22 A c B Fig. 6A-C. Induct ion of vari at ion in plants regenerated from embryo-derived callus of Triticum aestivum. A Callus obtained from imm ature embryo on tran sfer to IAA + BAP medium showing differentiation ofshoots and plantlets. B The same transferred to field. C Variation in the spikes obtained from B. (Bajaj 1986a) 23 Somaclonal Va riation 70 60 50 Embryo-derived callus 40 30 2 E!.

Anther culture has generated a wide range of genetic variability which has resulted in the release of varieties of wheat and rice (Hu 1986; Loo and Xu 1986; de Buyser et al. 1987). Attempts to obtain somatic hybrids in rice (Oryza sativa X Echinochloa oryziola) have recently yielded positive results (Terada et al. 1987a). The transformation studies on cereals have greatly advanced. and the recent success of the regeneration of complete maize and rice plants from transformed protoplasts (Rhodes et al.

And the values in parenthesis indicate the fresh weight (mg) of largest colony formed in each treatment Som aclonal Variation 15 Fig. 3A·F. Screening of variation in cell suspensions of Vigna radiata for tolerance of Na CI. 4-D (2 mg /l) with O. I. and 2% NaCl respectively as observed after 7 weeks of incubation . Note highly reduced number and size of the resistant colonies developing on 2% salt. 4 -D (2 mg /l) with O. I. and 2% salt respectively as observed after 7 weeks of incubation. (Gosal and Bajaj 1984) Furthermore.

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