Structure-based study of viral replication: with CD-ROM by R Holland Cheng, Tatsuo Miyamura, R Holland Cheng, Tatsuo

By R Holland Cheng, Tatsuo Miyamura, R Holland Cheng, Tatsuo Miyamura

Human rhinovirus phone access and uncoating -- position of lipid microdomains in influenza virus multiplication -- capabilities of integrin alpha2beta1, a collagen receptor, within the internalization of echovirus-- access mechanism of murine and SARS coronaviruses: similarity and dissimilarity-- Hepatitis viruses, signaling occasions, and modulation of the innate host reaction -- Virus-cell interplay of HCV -- RNA replication of hepatitis C virus -- constitution and dynamics in viral RNA packaging -- Rational layout of viral protein constructions with predetermined immunological homes -- Bioinformatics assets for the examine of viruses on the Virginia Bioinformatics Institute -- Virus structure probed by way of atomic strength microscopy -- Filovirus meeting and budding -- demanding situations in designing HIV Env immunogens for constructing a vaccine -- Insights into the caliciviridae kinfolk -- Mathematical techniques for stoichiometric quantification in experiences of viral meeting and DNA packaging -- Virus-like debris of fish nodavirus -- The meeting of the double-layered capsids of phytoreoviruses -- constitution and meeting of human herpesviruses: new insights from cryo-electron microscopy and tomography -- Human papillomavirus style sixteen capsid proteins: immunogenicity and attainable use as prophylactic vaccine antigens -- Chimeric recombinant Hepatitis E virus-like debris providing international epitopes as a singular vector of vaccine via oral management -- Nucleocapsid protein of hantaviruses

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16. , Sherman, Y. A. (1992). J. , 119,493. 38 2: Morphological recognition of apoptosis 17. A. (1993). J. PathoL, 170,1. 18. , Cornelisse, CJ. H. (1993). J. Histochem. , 41,7. 19. , Ohyama, H. and Yamada, T. (1997). Histochem. , 29,823. 20. , Nakayama, K. A. (1994). J. , 108,2261. 21. , Ansari, B. and Hopwood, D. (1994). J. , 107, 3569. 22. S. D. (1977). Ultrastruct. , 60, 272. 23. , Hvala, M. and Chun, J. (1998). , 5,702. 24. , Bultinck, J. and Herman, A. (1996). Atherosclerosis, 120,115 25. M.

G. c-myc) (Oncor) . EcoRI (Gibco BRL) • Hindlll (Gibco BRL) . 2 M Method 1. 5 U/ug DNA) for 8 h 37 °C. 2. Treat the reaction mixture with 2 units of DNase-free RNase for 1 h at 37°C. 3. Extract the samples with organic solvents as described in part A (step 3). 4. 2 M NaCI and 100% ethanol, wash the pellets with 70% ethanol, aspirate the supernatant, and air-dry the pellet. 5. Dissolve the pellet in TE buffer and quantitate using a spectrophotometer. 6. Electrophorese 10 ug of the DNA sample as described in Chapter 3, Protocol 2.

It is recommended that workers determine empirically the conditions required for optimal staining of their own tissue samples. This technique follows the protocol in the ApopTag® kit handbook and uses the majority of the reagents supplied by the kit (product code S7100, Oncor). Listed below are the specific modifications we have made to the standard kit protocol. Reagents • proteinase K . PBS . TdT Method 11. Dewax and rehydrate sections as detailed in Protocol 7. 12. Incubate sections with proteinase K in PBS (40 ug/ml) for 20 min.

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