Transcription Factors: Methods and Protocols by Céline Charlot, Hélène Dubois-Pot, Tsvetan Serchov, Yves

By Céline Charlot, Hélène Dubois-Pot, Tsvetan Serchov, Yves Tourrette, Bohdan Wasylyk (auth.), Paul J. Higgins (eds.)

In the previous couple of years, major breakthroughs in transcription study extended our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription elements: tools and Protocols, specialists within the box describe state of the art methods that investigators can use to probe serious mechanisms underlying transcription issue nuclear-cytoplasmic trafficking in addition to to evaluate the useful impression of post-translational transformations on transcription issue functionality. The chapters are written via well-liked scientists, a lot of whom constructed those equipment, and spotlight protocols that target particular transcription issue relatives with specific relevance to human sickness. Composed within the hugely winning Methods in Molecular Biology™ sequence layout, every one bankruptcy features a short advent, step by step tools, a listing of invaluable fabrics, and a Notes part which stocks pointers on troubleshooting and heading off identified pitfalls.

Comprehensive and present, Transcription components: tools and Protocols compiles the newest thoughts for elucidating controls on transcription issue intracellular localization and job, and hence is not like the other methods-based textual content on transcriptional legislation this day.

Show description

Read Online or Download Transcription Factors: Methods and Protocols PDF

Similar biology books

Receptor Binding Techniques

Holding the excessive criteria set via the profitable first variation, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical and academia reap the benefits of the newest advancements to supply certain sensible equipment for learning receptors in silico, in vitro, and in vivo.

Angiogenesis: In Vitro Systems Part A

Angiogenesis is the expansion of recent blood vessels and is a vital normal technique within the physique. A fit physique continues an ideal stability of angiogenesis modulators. in lots of severe illness states, even if, the physique loses keep an eye on over antiogenesis. ailments which are angiogensis-dependent outcome while blood vessels both develop excessively or insufficiently.

Systems Biology and Regulatory Genomics: Joint Annual RECOMB 2005 Satellite Workshops on Systems Biology and on Regulatory Genomics, San Diego, CA, USA; December 2-4, 2005, Revised Selected Papers

This e-book constitutes the completely refereed post-proceedings of 2 joint RECOMB 2005 satellite tv for pc occasions: the 1st Annual Workshop on structures Biology, RSB 2005 and the second one Annual Workshop on Regulatory Genomics, RRG 2005, held in San Diego, CA, united states in December 2005. The 21 revised complete papers offered have been rigorously reviewed and chosen for inclusion within the booklet.

Additional resources for Transcription Factors: Methods and Protocols

Sample text

Hanson CA, Wood LD, Hiebert SW (2008) Cellular stress triggers TEL nuclear export via two genetically separable pathways. J Cell Biochem 104:488–498 25. Roukens MG, Alloul-Ramdhani M, Vertegaal AC, Anvarian Z, Balog CI, Deelder AM, Hensbergen PJ, Baker DA (2008) Identification of a new site of sumoylation on Tel (ETV6) uncovers a PIAS-dependent mode of regulating Tel function. Mol Cell Biol 28:2342–2357 26. Roukens MG, Alloul-Ramdhani M, Moghadasi S, Op den Brouw M, Baker DA (2008) Downregulation of vertebrate Tel (ETV6) and Drosophila Yan is facilitated by an evolutionarily conserved mechanism of F-box-mediated ubiquitination.

New Ets-related factor 2), which is involved in blood vessel development, is increased in a HIF-1a independent manner under hypoxia in human umbilical vein endothelial cells (95). Angiopoietin-1 protein, a growth factor that promotes angiogenesis, regulates this induction. Changes in Ets protein levels affect the hypoxic response in various ways. 2. Cooperation of Ets Factors and HIF in Hypoxic Induction of Target Genes HIF-1 and HIF-2 have been shown to interact with various transcription factors.

All these studies highlight the close link between hypoxia-inducible factors and Ets transcription 20 Charlot et al.  2. Ets factors in the hypoxic induction of target genes. Hypoxia activates HIF transcription factors which in turn cooperate with Ets-1 or Elk-1 to induce target genes. Hypoxia also activates Elk-1 (phosphorylation by MAPK) leading to induction of two target genes. Hypoxia enhances Net degradation, leading to the loss of the repression and induction of target genes. factors under hypoxic conditions, suggesting that Ets factors are involved in responses to oxygen in the tumor environment during tumorigenesis.

Download PDF sample

Rated 4.38 of 5 – based on 29 votes