Trends in Stem Cell Biology and Technology by Ariff Bongso, Chui-Yee Fong (auth.), Hossein Baharvand

By Ariff Bongso, Chui-Yee Fong (auth.), Hossein Baharvand (eds.)

The research of stem telephone examine has lately won the eye from a turning out to be, multidisciplinary group of scientist; this exponential progress of curiosity is pushed by way of the desire of gaining knowledge of therapies for a number of ailments via transplantation medication. developments in Stem Cells Biology and know-how aptly serves this constructing group because it unearths new elements of stem telephone learn via particularly masking stories taken with spermatogonial stem cells, uniparental embryonic stem mobile strains, the iteration of gametes from stem cells, reprogramming germ cells to stem cells, nuclear and somatic mobilephone genetic reprogramming, tissue engineering and mechanotransduction of stem cells and eventually the advance of stem phone applied sciences for the therapy of deafness, center sickness, corneal damage and diabetes. With contributions by means of best scientists and popular students, tendencies in Stem Cells Biology and expertise deals a large viewers innovative details at an important time during this ever increasing field.

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Banking on human embryonic stem cells: estimating the number of donor cell lines needed for HLA matching. Lancet 2005;366:2019–25. 8. Cibelli JB, Grant KA, Chapman KB, et al. Parthenogenetic stem cells in nonhuman primates. Science 2002;295:819. 9. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663–76. 10. Barton SC, Surani MA, Norris ML. Role of paternal and maternal genomes in mouse development.

An overview of the procedure is outlined in Fig. 3. Blastocyst stage embryos from which the zona pellucida has been removed, are placed in individual wells of a 96-well plate and are cultured for 3 days to form outgrowths. To disaggregate these outgrowths (passage 0), the whole well is treated with trypsin, and all cells from each well of the 96-well plate are transferred to a well of a 48-well plate. An alternative method to this is picking and disaggregating only the inner cell mass of the outgrowth (58, 61).

25. Reubinoff BE, Pera MF, Fong CY, Trounson A. Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 2000;18:399–404. 26. Richards M, Fong CY, Chan WK, Wong PC, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 2002;20:933–6. 27. Richards M, Tan S, Fong CY, Biswase A, Chan WK, Bongso A. Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells.

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