Vaccinia Virus and Poxvirology: Methods and Protocols by Stuart N. Isaacs

By Stuart N. Isaacs

A accomplished number of state-of-the-art easy methods to research and paintings with the vaccinia virus and different poxvirus gene transcriptions. those quite simply reproducible ideas can be utilized for the development and characterization of recombinant viruses; for the research of poxvirus gene transcription and DNA replication; for the research of the binding, access, and move of the virus in host cells; and for using the virus in immunologic and cell-cell fusion assays. different tools conceal poxvirus bioinformatics, points of viral pathogenesis at either a protein and an animal version point, and the examine of immune responses to poxviruses-the latter a severe skill given the $64000 function of vaccinia virus in smallpox vaccination and their strength position as vaccine vectors directed opposed to infectious brokers and melanoma.

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Occup. Environ. Med. 59, 573–574. 27. 27 Lane, J. , Ruben, F. , Neff, J. , and Millar, J. D. (1969) Complications of smallpox vaccination, 1968. National surveillance in the United States. N. Engl. J. Med. 281, 1201–1208. 28. 28 Lane, J. , Ruben, F. , Neff, J. , and Millar, J. D. (1970) Complications of smallpox vaccination, 1968: results of ten statewide surveys. J. Infect. Dis. 122, 303–309. 29. Advisory Committee on Dangerous Pathogens and Advisory Committee on Genetic Modifications. (1990) Vaccination of Laboratory Workers Handling Vaccinia and Related Poxviruses Infectious for Humans.

52, 1–28. 37. CDC. (2003) Supplemental recommendations on adverse events following smallpox vaccine in the pre-event vaccination program: recommendations of the Advisory Committee on Immunization Practices. MMWR Morb. Mortal Wkly. Rep. 52, 282–284. 38. 38 Halsell, J. , Riddle, J. , Atwood, J. , Poland, G. , et al. (2003) Myopericarditis following smallpox vaccination among vaccinianaive US military personnel. JAMA 289, 3283–3289. 39. 39 Briody, B. A. (1959) Response of mice to ectromelia and vaccinia viruses.

Contemp Top Lab Anim Sci 41, 57–60. 43. Engler, R. , and Leung, D. Y. (2002) Smallpox vaccination: Risk considerations for patients with atopic dermatitis. J Allergy Clin Immunol 110, 357–365. 14 Isaacs Genetic Markers to Isolate Recombinants 15 2 Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers María M. Lorenzo, Inmaculada Galindo, and Rafael Blasco* Summary The standard approach for the isolation of vaccinia virus recombinants involves homologous recombination between a transfected plasmid and the replicating viral DNA.

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